Ccdc181 is a microtubule-binding protein that interacts with Hook1 in haploid male germ cells and localizes to the sperm tail and motile cilia.

ABSTRACT: Disruption of murine Hook1 results in a disturbed spermatogenesis and consequently leads to male infertility in mice. Within these mice abnormal sperm development starts with a disorganization of the microtubular manchette in elongating spermatids that leads to an abnormal head shape as well as to distinctive structural changes in the flagella of the sperm. To elucidate Hook1 function in male germ cell differentiation a yeast two-hybrid screen was performed using a murine testicular library, which leads to the identification of several putative Hook1 interacting proteins. One of the isolated cDNA fragments encodes for the coiled-coil domain containing protein 181 (Ccdc181). The putative interaction of Ccdc181 with Hook1 was verified by FRET analysis and interacting regions were identified

SUBMITTER: Schwarz T 

PROVIDER: S-EPMC5496667 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature