How to speed up the polymerase chain reaction.

ABSTRACT: Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

SUBMITTER: Bustin SA 

PROVIDER: S-EPMC5496742 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature