HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8⁺ T cells.

ABSTRACT: Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8⁺ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classical MHC I molecule HLA-E can present pathogen-derived peptides to CD8⁺ T cells. In this manuscript, we identified the antigen recognized by an HLA-E-restricted CD8⁺ T cell clone isolated from an Mtb latently infected individual as a peptide from the Mtb protein, MPT32. Recognition by the CD8⁺ T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene. This is the first description of a post-translationa

SUBMITTER: Harriff MJ 

PROVIDER: S-EPMC5496856 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature