Ribo-attenuators: novel elements for reliable and modular riboswitch engineering.

ABSTRACT: Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable

SUBMITTER: Folliard T 

PROVIDER: S-EPMC5496857 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature