Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content.

ABSTRACT: Coumarin and its derivatives are widely used as fragrances in industrial products and have medical value. The goal of the present study was to discover genes and pathways related to coumarin biosynthesis in Melilotus albus using transcriptome analysis. The genes of five M. albus near-isogenic lines (NILs) that had different coumarin content and β-glucosidase activity according to the investigation of pedigree were quantified and then analysed by RNA-Seq. Using transcriptome analysis, differentially expressed genes (DEGs) were identified in two pairwise comparisons that differed in coumarin content as well as in two pairwise comparisons that differed in β-glucosidase activity. Gene expression pattern analysis suggested similar transcriptional trends in the genotypes with the same coumarin l

SUBMITTER: Luo K 

PROVIDER: S-EPMC5496894 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature