Graphical Method for Choosing Optimized Conditions Given a Pump Pressure and a Particle Diameter in Liquid Chromatography.

ABSTRACT: The general limitations on liquid chromatographic performance in isocratic and gradient elution are now well understood. Many workers have contributed to this understanding and to developing graphical methods, or plots, to illustrate the capabilities of chromatographic systems over a wide range of values of operational parameters. These have been invaluable in getting a picture, in broad strokes, about the value of changing an operational parameter or the value of one separation approach over another. Here we present a plotting approach more appropriate for determining how to use chromatography most efficiently in one's own laboratory. The axes are linear: column length vertical and mobile phase velocity horizontal. In this coordinate system, straight lines with intercept zero correspond t

SUBMITTER: Groskreutz SR 

PROVIDER: S-EPMC5497305 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature