Aminoglycoside resistance of Pseudomonas aeruginosa in cystic fibrosis results from convergent evolution in the mexZ gene.

ABSTRACT:

Rationale

Aminoglycoside (AG) resistance of Pseudomonas aeruginosa in cystic fibrosis (CF) is associated with poorer clinical outcomes and is usually due to overexpression of the efflux pump MexXY. MexXY is regulated by mexZ, one of the most commonly mutated genes in CF P. aeruginosa isolates. Little is known about the evolutionary relationship between AG resistance, MexXY expression and mexZ mutations.

Objectives

To test the hypothesis that AG resistance in P. aeruginosa develops in parallel with higher MexXY expression and mexZ mutations.

Methods

CF P. aeruginosa isolates were compared for chronically infected (CI) adults, CI children and children with new infection.

Measurements

One P. aeruginosa isolate from each patient was analysed for mexZ mutations, mex

SUBMITTER: Prickett MH 

PROVIDER: S-EPMC5497499 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature