De novo design of protein homo-oligomers with modular hydrogen-bond network-mediated specificity.

ABSTRACT: In nature, structural specificity in DNA and proteins is encoded differently: In DNA, specificity arises from modular hydrogen bonds in the core of the double helix, whereas in proteins, specificity arises largely from buried hydrophobic packing complemented by irregular peripheral polar interactions. Here, we describe a general approach for designing a wide range of protein homo-oligomers with specificity determined by modular arrays of central hydrogen-bond networks. We use the approach to design dimers, trimers, and tetramers consisting of two concentric rings of helices, including previously not seen triangular, square, and supercoiled topologies. X-ray crystallography confirms that the structures overall, and the hydrogen-bond networks in particular, are nearly identical to the design

SUBMITTER: Boyken SE 

PROVIDER: S-EPMC5497568 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature