Whole genome sequencing distinguishes between relapse and reinfection in recurrent leprosy cases.

ABSTRACT:

Background

Since leprosy is both treated and controlled by multidrug therapy (MDT) it is important to monitor recurrent cases for drug resistance and to distinguish between relapse and reinfection as a means of assessing therapeutic efficacy. All three objectives can be reached with single nucleotide resolution using next generation sequencing and bioinformatics analysis of Mycobacterium leprae DNA present in human skin.

Methodology

DNA was isolated by means of optimized extraction and enrichment methods from samples from three recurrent cases in leprosy patients participating in an open-label, randomized, controlled clinical trial of uniform MDT in Brazil (U-MDT/CT-BR). Genome-wide sequencing of M. leprae was performed and the resultant sequence assemblies analyzed in silic

SUBMITTER: Stefani MMA 

PROVIDER: S-EPMC5498066 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature