Visualizing Temperature Mediated Activation of Gelsolin and Its Deactivation By Pip₂: A Saxs Based Study.

ABSTRACT: This is the first report describing temperature based initiation of gelsolin's F-actin depolymerization activity, even in absence of free Ca²⁺ or low pH. Small angle X-ray scattering (SAXS) and circular dichroism (CD) studies revealed that temperature in the range of 30-40 °C is capable of opening the G1 domain alone, as remaining domains are held together by the Ca²⁺-sensitive C-tail latch without any loss in the secondary structural content. Full opening of all domains of tail-less gelsolin, and retention of closed shape for G2-G6 gelsolin merely by heating, further substantiated our findings. The Ca²⁺/pH independent activity of gelsolin near physiological temperature brought out a query: whether gelsolin is always active, and if not, what might deactivat

SUBMITTER: Badmalia MD 

PROVIDER: S-EPMC5498565 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature