Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish.

ABSTRACT: Neutrophil recruitment to injured tissue appears to be an evolutionarily conserved strategy for organisms to fight against exogenous insults. Recent studies have shown rhythmic migration of neutrophils and several factors, including melatonin, have been implicated in regulating this rhythmic migration. The mechanisms underlying how endogenous melatonin regulates rhythmic neutrophils migration, however, are unclear. Here we generated a zebrafish annat2 mutant that lacks endogenous melatonin and, subsequently, a Tg(lyz:EGFP);aanat2 ^-/- transgenic line that allows for monitoring neutrophils migration visually in live zebrafish. We observed that migrating neutrophils are significantly reduced in aanat2 ^-/- mutant zebrafish under a light/dark condition, and the disrupted m

SUBMITTER: Ren DL 

PROVIDER: S-EPMC5498597 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature