Identification of new MUC1 epitopes using HLA-transgenic animals: implication for immunomonitoring.

ABSTRACT:

Background

The success of immunotherapeutics in oncology and the search for further improvements has prompted revisiting the use of cancer vaccines. In this context, knowledge of the immunogenic epitopes and the monitoring of the immune response cancer vaccines generate are essential. MUC1 has been considered one of the most important tumor associated antigen for decades.

Methods

To identify HLA-restricted MUC1 peptides we used eight human MHC class I transgenic mouse lines, together covering more than 80% of the human population. MUC1 peptides were identified by vaccinating each line with full length MUC1 coding sequences and using an IFNγ ELIspot restimulation assay. Relevant peptides were tested in a flow cytometry-based tetramer assay and for their capacity to serve as t

SUBMITTER: Scheikl-Gatard T 

PROVIDER: S-EPMC5499006 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature