Development of a counterselectable seamless mutagenesis system in lactic acid bacteria.

ABSTRACT:

Background

Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To address this problem, several counterselectable markers have been developed, while few of them could be used in the wild-type host cells without pretreatment.

Results

The pheS gene encoding phenylalanyl-tRNA synthetase alpha subunit was identified in Lactococcus lactis NZ9000 genome. When mutant pheS gene (pheS*) under the control of the Lc. lactis NZ9000 L-lactate dehydrogenase promoter (P_ldh) was expressed from a plasmid, the resulted PheS* with an A312G substitution rend

SUBMITTER: Xin Y 

PROVIDER: S-EPMC5499019 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature