Tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal isolates from bubaline mastitis in Egypt.

ABSTRACT:

Aim

This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt.

Materials and methods

A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotypic and molecular methods (polymerase chain reaction [PCR]). CNS isolates were tested for tetracycline resistance using routine methods and multiplex PCR targeting tetracycline (tet) resistance genes followed by sequencing of positive PCR products and phylogenetic analysis.

Results

Isolation and identification of 28 (34.5%) CNS from normal and SCM buffaloes' milk, namely,

SUBMITTER: El-Razik KAA 

PROVIDER: S-EPMC5499090 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature