Assessment of Single Cell RNA-Seq Normalization Methods.

ABSTRACT: We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in External RNA Control Consortium (ERCC) RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data.

SUBMITTER: Ding B 

PROVIDER: S-EPMC5499114 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature