Systematic Analysis of the DNA Damage Response Network in Telomere Defective Budding Yeast.

ABSTRACT: Functional telomeres are critically important to eukaryotic genetic stability. Scores of proteins and pathways are known to affect telomere function. Here, we report a series of related genome-wide genetic interaction screens performed on budding yeast cells with acute or chronic telomere defects. Genetic interactions were examined in cells defective in Cdc13 and Stn1, affecting two components of CST, a single stranded DNA (ssDNA) binding complex that binds telomeric DNA. For comparison, genetic interactions were also examined in cells with defects in Rfa3, affecting the major ssDNA binding protein, RPA, which has overlapping functions with CST at telomeres. In more complex experiments, genetic interactions were measured in cells lacking EXO1 or RAD9, affecting different aspe

SUBMITTER: Holstein EM 

PROVIDER: S-EPMC5499144 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature