Environment-dependent regulation of spliceosome activity by the LSM2-8 complex in Arabidopsis.

ABSTRACT: Spliceosome activity is tightly regulated to ensure adequate splicing in response to internal and external cues. It has been suggested that core components of the spliceosome, such as the snRNPs, would participate in the control of its activity. The experimental indications supporting this proposition, however, remain scarce, and the operating mechanisms poorly understood. Here, we present genetic and molecular evidence demonstrating that the LSM2-8 complex, the protein moiety of the U6 snRNP, regulates the spliceosome activity in Arabidopsis, and that this regulation is controlled by the environmental conditions. Our results show that the complex ensures the efficiency and accuracy of constitutive and alternative splicing of selected pre-mRNAs, depending on the conditions. Moreover, miss-

SUBMITTER: Carrasco-Lopez C 

PROVIDER: S-EPMC5499552 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature