Bidirectional terminators in Saccharomyces cerevisiae prevent cryptic transcription from invading neighboring genes.

ABSTRACT: Transcription can be quite disruptive for chromatin so cells have evolved mechanisms to preserve chromatin integrity during transcription, thereby preventing the emergence of cryptic transcripts from spurious promoter sequences. How these transcripts are regulated and processed remains poorly characterized. Notably, very little is known about the termination of cryptic transcripts. Here, we used RNA-Seq to identify and characterize cryptic transcripts in Spt6 mutant cells (spt6-1004) in Saccharomyces cerevisiae. We found polyadenylated cryptic transcripts running both sense and antisense relative to genes in this mutant. Cryptic promoters were enriched for TATA boxes, suggesting that the underlying DNA sequence defines the location of cryptic promoters. While intragenic sense cryptic trans

SUBMITTER: Uwimana N 

PROVIDER: S-EPMC5499651 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature