Enrichment methods provide a feasible approach to comprehensive and adequately powered investigations of the brain methylome.

ABSTRACT: Methylome-wide association studies are typically performed using microarray technologies that only assay a very small fraction of the CG methylome and entirely miss two forms of methylation that are common in brain and likely of particular relevance for neuroscience and psychiatric disorders. The alternative is to use whole genome bisulfite (WGB) sequencing but this approach is not yet practically feasible with sample sizes required for adequate statistical power. We argue for revisiting methylation enrichment methods that, provided optimal protocols are used, enable comprehensive, adequately powered and cost-effective genome-wide investigations of the brain methylome. To support our claim we use data showing that enrichment methods approximate the sensitivity obtained with WGB methods and

SUBMITTER: Chan RF 

PROVIDER: S-EPMC5499761 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature