Temperature-dependent regulation of rDNA condensation in Saccharomyces cerevisiae.

ABSTRACT: Chromatin condensation during mitosis produces detangled and discrete DNA entities required for high fidelity sister chromatid segregation during mitosis and positions DNA away from the cleavage furrow during cytokinesis. Regional condensation during G1 also establishes a nuclear architecture through which gene transcription is regulated but remains plastic so that cells can respond to changes in nutrient levels, temperature and signaling molecules. To date, however, the potential impact of this plasticity on mitotic chromosome condensation remains unknown. Here, we report results obtained from a new condensation assay that wildtype budding yeast cells exhibit dramatic changes in rDNA conformation in response to temperature. rDNA hypercondenses in wildtype cells maintained at 37°C, compare

SUBMITTER: Shen D 

PROVIDER: S-EPMC5499843 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature