Distinct cis-acting regions control six6 expression during eye field and optic cup stages of eye formation.

ABSTRACT: The eye field transcription factor, Six6, is essential for both the early (specification and proliferative growth) phase of eye formation, as well as for normal retinal progenitor cell differentiation. While genomic regions driving six6 optic cup expression have been described, the sequences controlling eye field and optic vesicle expression are unknown. Two evolutionary conserved regions 5' and a third 3' to the six6 coding region were identified, and together they faithfully replicate the endogenous X. laevis six6 expression pattern. Transgenic lines were generated and used to determine the onset and expression patterns controlled by the regulatory regions. The conserved 3' region was necessary and sufficient for eye field and optic vesicle expression. In contrast, the two conserved enha

SUBMITTER: Ledford KL 

PROVIDER: S-EPMC5500183 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature