High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE.

ABSTRACT: Ion channels regulate a variety of physiological processes and represent an important class of drug target. Among the many methods of studying ion channel function, patch clamp electrophysiology is considered the gold standard by providing the ultimate precision and flexibility. However, its utility in ion channel drug discovery is impeded by low throughput. Additionally, characterization of endogenous ion channels in primary cells remains technical challenging. In recent years, many automated patch clamp (APC) platforms have been developed to overcome these challenges, albeit with varying throughput, data quality and success rate. In this study, we utilized SyncroPatch 768PE, one of the latest generation APC platforms which conducts parallel recording from two-384 modules with giga-seal d

SUBMITTER: Li T 

PROVIDER: S-EPMC5500279 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature