Three distinct pneumotypes characterize the microbiome of the lung in BALB/cJ mice.

ABSTRACT: Bacteria can rarely be isolated from normal healthy lungs using conventional culture techniques, supporting the traditional belief that the lungs are sterile. Yet recent studies using next generation sequencing report that bacterial DNA commonly found in the upper respiratory tract (URT) is present at lower levels in the lungs. Interpretation of that finding is complicated by the technical limitations and potential for contamination introduced when dealing with low biomass samples. The current work sought to overcome those limitations to clarify the number, type and source of bacteria present in the lungs of normal mice. Results showed that the oral microbiome is diverse and highly conserved whereas murine lung samples fall into three distinct patterns. 33% of the samples were sterile, as

SUBMITTER: Scheiermann J 

PROVIDER: S-EPMC5500332 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature