Comparison of quantitative real-time PCR and direct immunofluorescence for the detection of Pneumocystis jirovecii.

ABSTRACT:

Background

Pneumocystis pneumonia (PCP) is a serious risk for HIV-positive patients. Asymptomatic infection or colonisation with P. jirovecii has been shown to occur frequently. PCR assays frequently identify such cases, due to their high sensitivity. Quantitative real-time PCR (qPCR) gene copy number cut-off values have been suggested to differentiate colonisation and infection; these need to be standardised for routine use. We compared the results of qPCR with an immunofluorescence assay (IFA) to determine a specific cut-off value.

Methods

From March 2005 through June 2009, induced sputum specimens were collected from adult patients who were clinically suspected of having PCP, at the Chris Hani Baragwanath Hospital in Gauteng, South Africa. Laboratory diagnosis of PCP was

SUBMITTER: Moodley B 

PROVIDER: S-EPMC5500343 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature