Soil bacterial quantification approaches coupling with relative abundances reflecting the changes of taxa.

ABSTRACT: Understanding the abundance change of certain bacterial taxa is quite important for the study of soil microbiology. However, the observed differences of relative abundances by high-throughput techniques may not accurately reflect those of the actual taxon abundances. This study investigated whether soil microbial abundances coupling with microbial quantities can be more informative in describing the microbial population distribution under different locations. We analyzed relative abundances of the major species in soil microbial communities from Beijing and Tibet grasslands by using 16 S rRNA high-throughput sequencing technique, and quantified the absolute bacterial cell numbers directly or indirectly by multiple culture-independent measurements, including adenosine tri-phosphate (ATP), f

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC5500469 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature