Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge.

ABSTRACT: Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to

SUBMITTER: Gallagher-Jones M 

PROVIDER: S-EPMC5500580 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature