A Metagenomic Analysis Provides a Culture-Independent Pathogen Detection for Atopic Dermatitis.

ABSTRACT:

Purpose

Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested a successive identification of AD-associated microbes, followed by a culture-independent serum detection of the identified microbe.

Methods

A total of 43 genomic DNA preparations from washing fluid of the cubital fossa of 6 healthy controls, skin lesions of 27 AD patients, 10 of which later received treatment (post-treatment), were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform.

Results

Microbial diversity was decreased in AD, and was restored following treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, and Streptococcus, whereas Alcaligenaceae (f), Sediminibacteriu

SUBMITTER: Kim MH 

PROVIDER: S-EPMC5500701 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature