Quantitative microspectroscopic imaging reveals viral and cellular RNA helicase interactions in live cells.

ABSTRACT: Human cells detect RNA viruses through a set of helicases called RIG-I-like receptors (RLRs) that initiate the interferon response via a mitochondrial signaling complex. Many RNA viruses also encode helicases, which are sometimes covalently linked to proteases that cleave signaling proteins. One unresolved question is how RLRs interact with each other and with viral proteins in cells. This study examined the interactions among the hepatitis C virus (HCV) helicase and RLR helicases in live cells with quantitative microspectroscopic imaging (Q-MSI), a technique that determines FRET efficiency and subcellular donor and acceptor concentrations. HEK293T cells were transfected with various vector combinations to express cyan fluorescent protein (CFP) or YFP fused to either biologically active HC

SUBMITTER: Corby MJ 

PROVIDER: S-EPMC5500786 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature