Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.

ABSTRACT: The oligosaccharyltransferase complex, localized in the endoplasmic reticulum (ER) of eukaryotic cells, is responsible for the N-linked glycosylation of numerous protein substrates. The membrane protein STT3 is a highly conserved part of the oligosaccharyltransferase and likely contains the active site of the complex. However, understanding the catalytic determinants of this system has been challenging, in part because of a discrepancy in the structural topology of the bacterial versus eukaryotic proteins and incomplete information about the mechanism of membrane integration. Here, we use a glycosylation mapping approach to investigate these questions. We measured the membrane integration efficiency of the mouse STT3-A and yeast Stt3p transmembrane domains (TMDs) and report a

SUBMITTER: Lara P 

PROVIDER: S-EPMC5500801 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature