Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

ABSTRACT: Abnormal development of multiciliated cells is a hallmark of a variety of human conditions associated with chronic airway diseases, hydrocephalus and infertility. Multiciliogenesis requires both activation of a specialized transcriptional program and assembly of cytoplasmic structures for large-scale centriole amplification that generates basal bodies. It remains unclear, however, what mechanism initiates formation of these multiprotein complexes in epithelial progenitors. Here we show that this is triggered by nucleocytoplasmic translocation of the transcription factor E2f4. After inducing a transcriptional program of centriole biogenesis, E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins w

SUBMITTER: Mori M 

PROVIDER: S-EPMC5500891 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature