TnseqDiff: identification of conditionally essential genes in transposon sequencing studies.

ABSTRACT:

Background

Tn-Seq is a high throughput technique for analysis of transposon mutant libraries to determine conditional essentiality of a gene under an experimental condition. A special feature of the Tn-seq data is that multiple mutants in a gene provides independent evidence to prioritize that gene as being essential. The existing methods do not account for this feature or rely on a high-density transposon library. Moreover, these methods are unable to accommodate complex designs.

Results

The method proposed here is specifically designed for the analysis of Tn-Seq data. It utilizes two steps to estimate the conditional essentiality for each gene in the genome. First, it collects evidence of conditional essentiality for each insertion by comparing read counts of that insertio

SUBMITTER: Zhao L 

PROVIDER: S-EPMC5500955 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature