HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

ABSTRACT: Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

SUBMITTER: Mumbach MR 

PROVIDER: S-EPMC5501173 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature