Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout.

ABSTRACT: Cell-based assays are finding wider use in evaluating compounds in primary screens for drug development, yet it is still challenging to measure enzymatic activities as an end point in a cell-based assay. This paper reports a strategy that combines state-of-the-art cantilever free polymer pen lithography (PPL) with self-assembled monolayer laser desorption-ionization (SAMDI) mass spectrometry to guide cell localization and measure cellular enzymatic activities. Experiments are conducted with a 384 spot array, in which each spot is composed of ∼400 nanoarrays and each array has a 10 × 10 arrangement of 750 nm features that present extracellular matrix (ECM) proteins surrounded by an immobilized phosphopeptide. Cells attach to the individual nanoarrays, where they can be cultured and treated

SUBMITTER: Cabezas MD 

PROVIDER: S-EPMC5501326 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature