Defined Engineered Human Myocardium With Advanced Maturation for Applications in Heart Failure Modeling and Repair.

ABSTRACT:

Background

Advancing structural and functional maturation of stem cell-derived cardiomyocytes remains a key challenge for applications in disease modeling, drug screening, and heart repair. Here, we sought to advance cardiomyocyte maturation in engineered human myocardium (EHM) toward an adult phenotype under defined conditions.

Methods

We systematically investigated cell composition, matrix, and media conditions to generate EHM from embryonic and induced pluripotent stem cell-derived cardiomyocytes and fibroblasts with organotypic functionality under serum-free conditions. We used morphological, functional, and transcriptome analyses to benchmark maturation of EHM.

Results

EHM demonstrated important structural and functional properties of postnatal myocardium, includ

SUBMITTER: Tiburcy M 

PROVIDER: S-EPMC5501412 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature