Tissue Factor Prothrombotic Activity Is Regulated by Integrin-arf6 Trafficking.

ABSTRACT:

Objective

Coagulation initiation by tissue factor (TF) is regulated by cellular inhibitors, cell surface availability of procoagulant phosphatidylserine, and thiol-disulfide exchange. How these mechanisms contribute to keeping TF in a noncoagulant state and to generating prothrombotic TF remain incompletely understood.

Approach and results

Here, we study the activation of TF in primary macrophages by a combination of pharmacological, genetic, and biochemical approaches. We demonstrate that primed macrophages effectively control TF cell surface activity by receptor internalization. After cell injury, ATP signals through the purinergic receptor P2rx7 induce release of TF⁺ microvesicles. TF cell surface availability for release onto microvesicles is regulated by the

SUBMITTER: Rothmeier AS 

PROVIDER: S-EPMC5501484 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature