Alpha 2 Na⁺,K⁺-ATPase silencing induces loss of inflammatory response and ouabain protection in glial cells.

ABSTRACT: Ouabain (OUA) is a cardiac glycoside that binds to Na⁺,K⁺-ATPase (NKA), a conserved membrane protein that controls cell transmembrane ionic concentrations and requires ATP hydrolysis. At nM concentrations, OUA activates signaling pathways that are not related to its typical inhibitory effect on the NKA pump. Activation of these signaling pathways protects against some types of injury of the kidneys and central nervous system. There are 4 isoforms of the alpha subunit of NKA, which are differentially distributed across tissues and may have different physiological roles. Glial cells are important regulators of injury and inflammation in the brain and express the α1 and α2 NKA isoforms. This study investigated the role of α2 NKA in OUA modulation of the neuroinflammatory

SUBMITTER: Kinoshita PF 

PROVIDER: S-EPMC5501845 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature