Computational sensing of herpes simplex virus using a cost-effective on-chip microscope.

ABSTRACT: Caused by the herpes simplex virus (HSV), herpes is a viral infection that is one of the most widespread diseases worldwide. Here we present a computational sensing technique for specific detection of HSV using both viral immuno-specificity and the physical size range of the viruses. This label-free approach involves a compact and cost-effective holographic on-chip microscope and a surface-functionalized glass substrate prepared to specifically capture the target viruses. To enhance the optical signatures of individual viruses and increase their signal-to-noise ratio, self-assembled polyethylene glycol based nanolenses are rapidly formed around each virus particle captured on the substrate using a portable interface. Holographic shadows of specifically captured viruses that are surrounded

SUBMITTER: Ray A 

PROVIDER: S-EPMC5501859 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature