Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding.

ABSTRACT:

Background

Retrotransposons play a central role in plant evolution and could be a powerful endogenous source of genetic and epigenetic variability for crop breeding. To ensure genome integrity several silencing mechanisms have evolved to repress retrotransposon mobility. Even though retrotransposons fully depend on transcriptional activity of the host RNA polymerase II (Pol II) for their mobility, it was so far unclear whether Pol II is directly involved in repressing their activity.

Results

Here we show that plants defective in Pol II activity lose DNA methylation at repeat sequences and produce more extrachromosomal retrotransposon DNA upon stress in Arabidopsis and rice. We demonstrate that combined inhibition of both DNA methylation and Pol II activity leads to a strong

SUBMITTER: Thieme M 

PROVIDER: S-EPMC5501947 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature