Massively parallel nanowell-based single-cell gene expression profiling.

ABSTRACT:

Background

Technological advances have enabled transcriptome characterization of cell types at the single-cell level providing new biological insights. New methods that enable simple yet high-throughput single-cell expression profiling are highly desirable.

Results

Here we report a novel nanowell-based single-cell RNA sequencing system, ICELL8, which enables processing of thousands of cells per sample. The system employs a 5,184-nanowell-containing microchip to capture ~1,300 single cells and process them. Each nanowell contains preprinted oligonucleotides encoding poly-d(T), a unique well barcode, and a unique molecular identifier. The ICELL8 system uses imaging software to identify nanowells containing viable single cells and only wells with single cells are processed into

SUBMITTER: Goldstein LD 

PROVIDER: S-EPMC5501953 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature