DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.

ABSTRACT: DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.

SUBMITTER: Dai N 

PROVIDER: S-EPMC5501962 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature