UV-activated multilayer nanomatrix provides one-step tunable carbohydrate structural characterization in MALDI-MS.

ABSTRACT: The structure-specific fragmentation of gas-phase ions in tandem mass spectrometry among other techniques provides an efficient analytical method for confirming unknown analytes or for elucidating chemical structures. Using concentration-dependent UV-absorbing matrix-functionalized magnetic nanoparticles and matrix-assisted laser desorption-ionization mass spectrometry (MALDI MS), we developed a single-step pseudo-MS/MS approach for tunable ionization and fragmentation to facilitate structure determination. Without chemical derivatization, we have demonstrated that this approach successfully distinguished isomeric sets of di-, tri- and tetrasaccharides. Low concentration of nanomatrix provided an enhanced signal for accurate mass determination of the intact molecular ions of analytes prese

SUBMITTER: Obena RP 

PROVIDER: S-EPMC5502396 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature