Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets.

ABSTRACT: CD177 presents antigens in allo- and autoimmune diseases on the neutrophil surface. Individuals can be either CD177-deficient or harbor distinct CD177^neg and CD177^pos neutrophil subsets. We studied mechanisms controlling subset-restricted CD177 expression in bimodal individuals. CD177^pos, but not CD177^neg neutrophils, produced CD177 protein and mRNA. Haplotype analysis indicated a unique monoallelic CD177 expression pattern, where the offspring stably transcribed either the maternal or paternal allele. Hematopoietic stem cells expressed both CD177 alleles and silenced one copy during neutrophil differentiation. ChIP and reporter assays in HeLa cells with monoallelic CD177 expression showed that methylation reduced reporter ac

SUBMITTER: Eulenberg-Gustavus C 

PROVIDER: S-EPMC5502425 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature