Recombinant silicateins as model biocatalysts in organosiloxane chemistry.

ABSTRACT: The family of silicatein enzymes from marine sponges (phylum Porifera) is unique in nature for catalyzing the formation of inorganic silica structures, which the organisms incorporate into their skeleton. However, the synthesis of organosiloxanes catalyzed by these enzymes has thus far remained largely unexplored. To investigate the reactivity of these enzymes in relation to this important class of compounds, their catalysis of Si-O bond hydrolysis and condensation was investigated with a range of model organosilanols and silyl ethers. The enzymes' kinetic parameters were obtained by a high-throughput colorimetric assay based on the hydrolysis of 4-nitrophenyl silyl ethers. These assays showed unambiguous catalysis with k_cat/K_m values on the order of 2-5

SUBMITTER: Tabatabaei Dakhili SY 

PROVIDER: S-EPMC5502584 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature