A Series of Suppressive Signals within the Drosophila Circadian Neural Circuit Generates Sequential Daily Outputs.

ABSTRACT: We studied the Drosophila circadian neural circuit using whole-brain imaging in vivo. Five major groups of pacemaker neurons display synchronized molecular clocks, yet each exhibits a distinct phase of daily Ca²⁺ activation. Light and neuropeptide pigment dispersing factor (PDF) from morning cells (s-LNv) together delay the phase of the evening (LNd) group by ∼12 hr; PDF alone delays the phase of the DN3 group by ∼17 hr. Neuropeptide sNPF, released from s-LNv and LNd pacemakers, produces Ca²⁺ activation in the DN1 group late in the night. The circuit also features negative feedback by PDF to truncate the s-LNv Ca²⁺ wave and terminate PDF release. Both PDF and sNPF suppress basal Ca²⁺ levels in target pacemakers with long durations by cell-autonom

SUBMITTER: Liang X 

PROVIDER: S-EPMC5502710 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature