Early Detection of Cancer in Blood Using Single-Cell Analysis: A Proposal.

ABSTRACT: Here, we explore the potential of single-cell genomic analysis in blood for early detection of cancer; we consider a method that screens the presence of recurrent patterns of copy number (CN) alterations using sparse single-cell sequencing. We argue for feasibility, based on in silico analysis of existing single-cell data and cancer CN profiles. Sampling procedures from existing diploid single cells can render data for a cell with any given profile. Sampling from multiple published tumor profiles can interrogate cancer clonality via an algorithm that tests the multiplicity of close pairwise similarities among single-cell cancer genomes. The majority of common solid cancers would be detectable in this manner. As any early detection method must be verifiable and actionable, we describe how f

SUBMITTER: Krasnitz A 

PROVIDER: S-EPMC5502711 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature