SOX17 regulates cholangiocyte differentiation and acts as a tumor suppressor in cholangiocarcinoma.

ABSTRACT:

Background & aims

Cholangiocarcinoma (CCA) is a biliary malignancy linked to genetic and epigenetic abnormalities, such as hypermethylation of SOX17 promoter. Here, the role of SOX17 in cholangiocyte differentiation and cholangiocarcinogenesis was studied.

Methods

SOX17 expression/function was evaluated along the differentiation of human induced pluripotent stem cells (iPSC) into cholangiocytes, in the dedifferentiation process of normal human cholangiocytes (NHC) in culture and in cholangiocarcinogenesis. Lentiviruses for SOX17 overexpression or knockdown were used. Gene expression and DNA methylation profiling were performed.

Results

SOX17 expression is induced in the last stage of cholangiocyte differentiation from iPSC and regulates the acquisition of biliary mark

SUBMITTER: Merino-Azpitarte M 

PROVIDER: S-EPMC5502751 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature