Hyperpolarized δ-[1-¹³ C]gluconolactone as a probe of the pentose phosphate pathway.

ABSTRACT: The pentose phosphate pathway (PPP) is thought to be upregulated in trauma (to produce excess NADPH) and in cancer (to provide ribose for nucleotide biosynthesis), but simple methods for detecting changes in flux through this pathway are not available. MRI of hyperpolarized ¹³ C-enriched metabolites offers considerable potential as a rapid, non-invasive tool for detecting changes in metabolic fluxes. In this study, hyperpolarized δ-[1-¹³ C]gluconolactone was used as a probe to detect flux through the oxidative portion of the pentose phosphate pathway (PPP_ox ) in isolated perfused mouse livers. The appearance of hyperpolarized (HP) H¹³ CO₃^- within seconds after exposure of livers to HP-δ-[1-¹³ C]gluconolactone demo

SUBMITTER: Moreno KX 

PROVIDER: S-EPMC5502806 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature