Proximal clustering between BK and Ca_V1.3 channels promotes functional coupling and BK channel activation at low voltage.

ABSTRACT: Ca_V-channel dependent activation of BK channels is critical for feedback control of both calcium influx and cell excitability. Here we addressed the functional and spatial interaction between BK and Ca_V1.3 channels, unique Ca_V1 channels that activate at low voltages. We found that when BK and Ca_V1.3 channels were co-expressed in the same cell, BK channels started activating near -50 mV, ~30 mV more negative than for activation of co-expressed BK and high-voltage activated Ca_V2.2 channels. In addition, single-molecule localization microscopy revealed striking clusters of Ca_V1.3 channels surrounding clusters of BK channels and forming a multi-channel complex both in a heterologous system and in rat hippocampal and sympathetic

SUBMITTER: Vivas O 

PROVIDER: S-EPMC5503510 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature