Folate-dependent methylation of septins governs ciliogenesis during neural tube closure.

ABSTRACT: Periconception maternal folic acid (vitamin B₉) supplementation can reduce the prevalence of neural tube defects (NTDs), although just how folates benefit the developing embryo and promote closing of the neural tube and other morphologic processes during development remains unknown. Folate contributes to a 1-carbon metabolism, which is essential for purine biosynthesis and methionine recycling and affects methylation of DNA, histones, and nonhistone proteins. Herein, we used animal models and cultured mammalian cells to demonstrate that disruption of the methylation pathway mediated by folate compromises normal neural tube closure (NTC) and ciliogenesis. We demonstrate that the embryos with NTD failed to adequately methylate septin2, a key regulator of cilium structure and funct

SUBMITTER: Toriyama M 

PROVIDER: S-EPMC5503710 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature