MiR-497∼195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1α activity.

ABSTRACT: A specific bone vessel subtype, strongly positive for CD31 and endomucin (CD31^hiEmcn^hi), is identified as coupling angiogenesis and osteogenesis. The abundance of type CD31^hiEmcn^hi vessels decrease during ageing. Here we show that expression of the miR-497∼195 cluster is high in CD31^hiEmcn^hi endothelium but gradually decreases during ageing. Mice with depletion of miR-497∼195 in endothelial cells show fewer CD31^hiEmcn^hi vessels and lower bone mass. Conversely, transgenic overexpression of miR-497∼195 in murine endothelium alleviates age-related reduction of type CD31^hiEmcn^hi vessels and bone loss. miR-497∼195 cluster maintains the endothelial Notch activity and HIF-1α stability via

SUBMITTER: Yang M 

PROVIDER: S-EPMC5504303 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature